Measles virus blind to its epithelial cell receptor remains virulent in rhesus monkeys but cannot cross the airway epithelium and is not shed
J. Clin. Invest. Vincent H.J. Leonard, et al. 118:2448 doi:10.1172/JCI35454 [Go to this article.]

Figure 5
MV infects well-differentiated airway epithelium basolaterally and in an EpR-dependent fashion. (A) MV infection quantified by biofluorescence imaging following apical (A) or basolateral (B) infection. Measurements were performed on days 1, 3, 5, and 7 after infection (DPI) as indicated. Fluorescence intensity of GFP is expressed as the radiance units per second of photons leaving a square centimeter of tissue and radiating into a solid angle of 1 steradian (photons/s/cm²/sr). The dashed line indicates the average values of the uninfected sample. Error bars indicate mean ± SD. (B) Confocal imaging of basolaterally infected human primary airway epithelial cells. The cells were infected with the indicated viruses at an MOI of 0.1. The bottom panel is a vertical section of the top panel. Scale bar: 100 μm. Arrows indicate uninfected basal cells. Confocal images were captured with a Bio-Rad Radiance 2100 multiphoton confocal microscope.