Abstract
Authors
Rimas V. Lukas, Ruochen Du, Harrshavasan Congivaram, Kathleen McCortney, Karan Dixit, Craig Horbinski, Margaret Schwartz, Raymond Lezon, Lauren Singer, Ditte Primdahl, Jigisha Thakkar, Amy B. Heimberger, Roger Stupp, Priya Kumthekar
Figure 1
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(A ) Kaplan-Meier survival curve, showing a median overall survival of 6.7 months (95% CI, 4.1–10.2 months). (B ) Representative spatial sequential immunofluorescence (SeqIF) images demonstrating heterogeneity of EGFR expression within GFAP+ glioblastoma. The white lines demarcate EGFR protein expression within the solid tumor region. Green arrows designate vessels. (C and D ) Representative spatial SeqIF images of GFAP+ tumor cell expression (pseudocolored orange) surrounding ACTA2+ (pseudocolored green) and CD31+ (pseudocolored white) vessels from 2 different tumors. (E and F ) Matched spatial SeqIF images of EGFR+ tumor cell expression (pseudocolored purple) surrounding ACTA2+ and CD31+ tumor vessels. (E ) Demonstrates homogeneous EGFR expression in proximity to the vessel, while (F ) shows minimal EGFR expression surrounding the tumor vasculature. Yellow dashed lines designate the perivascular diffusion penetration distance quantified at 40 μm. Scale bars: 500 μm (B ) and 100 μm (C –F ).
