(A) LTF expression was analyzed by immunofluorescence of matched tissue for n =22 samples from the RNA-Seq analysis to compare patients with the minor allele (C/G) (n = 10) and those with the major allele (G/G) (n = 12), with representative images of the data set shown as a whole view of the slide in the heatmap images (left column: yellow, increased density of staining). In a 20× image of the slide, cells are outlined in white, with nuclei marked by DAPI and LTF pseudocolored purple. Left column: Full slide scans, zoomed to see the entire section. Scale bar: 500 µM. LTF is pseudocolored from purple to yellow scaled from 0 to 0.2. Right column: Representative zoomed in regions of major allele (top) and minor allele (bottom), where nuclei marked with blue DAPI and LTF colored purple. Scale bar: 100 µM. (B) The average LTF expression (mean fluorescence intensity) of cells per sample was compared between each genotype and the negative secondary-only control (n = 10 C/G and n = 12 G/G). (C) The quantity of LTF⁺ cells per total area was measured and then compared between the C/G and G/G genotypes using unpaired t test (n = 10 C/G and n = 12 G/G). (D) The percent of LTF⁺ cells of the total cells per sample was compared between each genotype group and further subdivided into prognostic categories based on greater than or less than median overall survival (poor prognosis contains n = 6 G/G and n = 7 C/G, good prognosis contains n = 6 G/G and n = 3 C/G). (E) Staining for CD3 and CD8 markers to determine CD8⁺ T cell infiltration, with representative images of each genotype and prognosis group (CD3, yellow; CD8, red; DAPI, blue). Scale bar: 100 µM. (F and G) Percent of T cells (F) and CD8⁺ T cells (G) of total cells per sample comparing the genotype/prognosis categories only. (H) CD107a expression of CD8⁺ T cells from all samples was compared between prognoses. (I) CD107a expression of CD8⁺ T cells was compared between the C/G genotype and G/G genotype.