The endoplasmic reticulum autophagy receptor TEX264 drives epidermal differentiation and is dysregulated in Darier disease

Christopher J. Johnson; Afua Tiwaa; Arti Parihar; Edwin Antonio; Brooke D. Lorenz; Reeteka Kudallur; Aaron Ramonett; Anthony Coon; Mrinal K. Sarkar; Johann Gudjonsson; Cory L. Simpson

doi:10.1172/jci.insight.196831

The endoplasmic reticulum autophagy receptor TEX264 drives epidermal differentiation and is dysregulated in Darier disease

Christopher J. Johnson,¹ Afua Tiwaa,¹ Arti Parihar,¹ Edwin Antonio,² Brooke D. Lorenz,² Reeteka Kudallur,¹ Aaron Ramonett,³ Anthony Coon,⁴ Mrinal K. Sarkar,⁴ Johann Gudjonsson,⁴ and Cory L. Simpson¹

Published October 22, 2026 - More info

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Abstract

Differentiating keratinocytes break down their organelles and nuclei to become the compacted cornified layers of the epidermal barrier in a poorly understood catabolic process. Live confocal imaging of stratified human organotypic epidermis revealed endoplasmic reticulum (ER) fragmentation and lysosomal engulfment in the cornifying layers, where we found up-regulation of TEX264, a receptor that mediates selective autophagy of the ER (reticulophagy). TEX264 expression was increased by ER stress, which caused precocious cornification of organotypic epidermis. In undifferentiated keratinocytes, ectopic TEX264 was sufficient to induce ER fragments, while in highly differentiated keratinocytes, it accelerated ER elimination and nuclear shrinkage; these effects were abolished by mutating the LC3 interacting region required for its autophagic function. Knockout of TEX264 or inhibiting its activation disrupted the maturation of organotypic cultures, pointing to a critical role for reticulophagy in cornification. Finally, in patient biopsies and an organotypic model of Darier disease, a genetic cornification disorder linked to ER dysfunction, we found increased TEX264 in areas of premature cornification (dyskeratosis). Our results identified TEX264 as a key driver of late-stage epidermal differentiation and led us to propose a model of cornification in which keratinocytes activate selective autophagy receptors to orchestrate orderly organelle elimination during cutaneous barrier formation.

The endoplasmic reticulum autophagy receptor TEX264 drives epidermal differentiation and is dysregulated in Darier disease

Christopher J. Johnson,¹ Afua Tiwaa,¹ Arti Parihar,¹ Edwin Antonio,² Brooke D. Lorenz,² Reeteka Kudallur,¹ Aaron Ramonett,³ Anthony Coon,⁴ Mrinal K. Sarkar,⁴ Johann Gudjonsson,⁴ and Cory L. Simpson¹

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The endoplasmic reticulum autophagy receptor TEX264 drives epidermal differentiation and is dysregulated in Darier disease

Christopher J. Johnson,1 Afua Tiwaa,1 Arti Parihar,1 Edwin Antonio,2 Brooke D. Lorenz,2 Reeteka Kudallur,1 Aaron Ramonett,3 Anthony Coon,4 Mrinal K. Sarkar,4 Johann Gudjonsson,4 and Cory L. Simpson1

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Christopher J. Johnson,¹ Afua Tiwaa,¹ Arti Parihar,¹ Edwin Antonio,² Brooke D. Lorenz,² Reeteka Kudallur,¹ Aaron Ramonett,³ Anthony Coon,⁴ Mrinal K. Sarkar,⁴ Johann Gudjonsson,⁴ and Cory L. Simpson¹